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Environmental Sample DNA Extraction Kit



Further improvement in the ability to remove PCR inhibitors! High purity and high yields from a wide variety of environmental samples!


Features

Suppress DNA adsorption to solid surfaces (e.g. soil)
Use DNA adsorption inhibitors with contaminated DNA that is over 2 orders less than skimmed milk
Remove PCR inhibitors and collect DNA with a high purity
Simple operation with purification by magnetic beads
Safe because phenol-chloroform is not required
Crush a wide range of microorganisms with beads beating


■ Monitoring of useful bacteria
■ Microbial community structure analysis
■Measurement of crop pathogens
…and other applications!

A comparison with the products of other firms Real-time Quantitative PCR Method Results (Bacteria 16S rRNA Gene)

Equal or better measurement values were obtained for all the soil samples in comparison with the soil DNA extraction kits of the other companies.

Product Contents

(50 batches)
 ・Beads tubes
 ・Extraction Buffer
 ・Lysis Solution
 ・PP Solution
 ・MBs Solution
 ・Binding Solution
 ・ Washing Solution

Necessary equipment, tools and reagents

(Equipment)
Homogenizer (2ml tube compliant) Recommended: FastPrep Series (Made by MP Biomedicals) Beads Crusher μT-01 (Made by Taitec) Vortex-Genie 2 (Made by Scientific Industries) + Vortex Adapter (Made by MO BIO Laboratories)
Vortex mixer
Refrigerated centrifuge (Maximum centrifugal force of 14,000 x g or more)
Heat block or water bath

(Tools)
Magnetic stand Recommended: Promega Z5332,Z5342, etc. ・Micropipette and tip1.5ml tube and 2ml tube (Reagents) ・Ethanol Eluate (Sterilized water, TE buffer etc.)

Protocol

Protocol

(1) Add a soil sample (0.5g), extraction buffer and lysis solution to a beads tub, and then carry out the beads beating (30 to 45 seconds)

(2) Centrifuge (5 minutes) and supernatant collection

(3) Add PP solution, and then centrifuge (5 minutes) and supernatant collection

(4) Add MBs solution and binding solution, and then stirring, magnetic attraction and supernatant collection
(5) Add washing solution, and then stirring, magnetic attraction and supernatant collection

(6) Add ethanol solution, and then stirring, magnetic attraction and supernatant collection

(7) After air drying, add elution liquid, and then heat for 5 to 10 minutes at 65°C

(8) Magnetic attraction and supernatant collection → Total DNA

Kit Insert (Manual)

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For the development of human life science | Future-oriented Tradingcompany | Global marketing | Tokyo Future Style, Inc.