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Bioconjugations – Fina Biosolutions, LLC

Fina Biosolutions is a R&D company developing bioconjugation products and offering the services. Their products are used in many applications. We introduce their cataloged products and services.

(Fina Biosolutions’ web site is here)

 

Bioconjugation services and products

-Bioconjugation

-Special expertise in protein polysaccharide conjugate vaccines

-Protein and carbohydrate labeling

-Hybridomas, antibody purification and labeling

 


Product introduction


 

CRM197 and TTHc

EcoCRM™

EcoCRM, economical CRM from E. coli, will significantly reduce the cost of conjugate vaccines.

CRM197, a genetically detoxified diphtheria toxin, is widely used as a carrier protein in conjugate vaccines. Effective conjugate vaccines against Streptococcus pneumonaie, Haemophilus influenzae b and Neisseria meningitidis have been made using this carrier protein. Fina Biosolutions has developed a highly efficient Escherichia coli expression system for CRM197, along with a simple purification scheme.  We express soluble, properly folded CRM197 in the cytoplasm of E.coli and achieve expression yields of grams per liter.

References 

Characterization of EcoCRM, an E. coli Expressed CRM197 Conjugate Vaccine Carrier Protein

EcoCRM™ – Economical Recombinant CRM197 – Significantly reducing the cost of conjugate vaccines

EcoCRM™: Affordable CRM197 Vaccine Carrier Protein

 

Recombinant Tetanus Toxin Heavy Chain Fragment C (rTTHc)

Tetanus toxoid (TT) is frequently used as the carrier protein for conjugate vaccines, in addition to being a component of the DPT vaccine. TT contains very strong T cell epitopes. However, as a toxoided protein, many of the surface lysines are blocked by the toxoiding process. TT is not a uniform product. Each manufacturer has its own specific toxoiding and purification process. Tetanus tends to aggregate with age. Furthermore, TT is not generally affordable in the quantities needed for research and early clinical work.

Tetanus toxoid heavy chain fragment C (TTHc) is the C terminal fragment of the toxin. It is devoid of the toxin’s enzymatic activity but contains the binding site necessary for uptake of the toxin by neurons. Significantly, the majority of the antibody response to tetanus toxoid is against epitopes contained within TTHc.   Fragment C contains the powerful T cell epitopes of tetanus toxin without the toxicity.

Fina Biosolutions now offers recombinant TTHc (rTTHc), combining the advantages of the toxoid with the reproducibility of a recombinant protein. FinaBio expresses rTTHc as a properly folded, soluble protein in E. coli cytoplasm.

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Amino-Dextran

Amino-Dextran (AmDex, AECM-dextran) consists of dextran functionalized with primary amines. Our AmDex is prepared from dextran using a protocol yielding a stable, amino functionalized dextran that is superior to the more commonly used reductive amination methods. The molecular weight profile of Fina Biosolutions’ AmDex is essentially unchanged from the starting dextran, as determined by analytical size exclusion chromatography. The product is desalted and lyophilized. We do extensive processing and testing to ensure that free amino reagent is removed, resulting in a consistent product with minimal lot-to-lot variation.

We have found that many commercial lots of amino-dextran contain variable amounts of dextran and that the amine content is often incorrect. Furthermore, the actual polymer molecular weight distributions of these preparations can vary extensively from the indicated molecular weight. These issues can mean that optimization data is based on incorrect information. Lot-to-lot variations means that protocols may need to be revised when a new lot of AmDex is used.

We take care to ensure that we provide a consistent product. Each batch of amino-dextran polymer is evaluated for molecular weight distribution and for the amine content, expressed as amines/polymer as well as assayed to determine the weight percent dextran. A certificate of analysis is provided for each lot.

Amino-dextrans are available in molecular weights from 3.5 kDa to 2000 kDa in a variety of amine ratios. Custom polymers can be synthesized to match your needs.

 

Product list

AD(MW KDa)x(NH2)
Product # Size
3.5 kDa
AD3.5×3 100 mg, 500 mg
6 kDa
AD6x05 100 mg, 500 mg
10 kDa
AD10x05 100 mg, 500 mg
AD10x10
20 kDa
AD20x5 100 mg, 500 mg
AD20x10
AD20x20
40 kDa
AD40x10 100 mg, 500 mg
AD40x20
AD40x50
70 kDa
AD70x10 100 mg, 500 mg
AD70x25
AD70x50
110 kDa
AD110x10 100 mg, 500 mg
AD110x25
AD110x50
AD110x100
150 kDa
AD150x25 100 mg, 500 mg
AD150x50
AD150x100
250 kDa
AD250x10 100 mg, 500 mg
AD250x25
AD250x50
AD250x100
AD250x150
500 kDa
AD500x25 100 mg, 500 mg
AD500x50
AD500x100
AD500x250
AD500x400
2000 kDa
AD2000x100 100 mg, 500 mg
AD2000x250
AD2000x500
AD2000x750
AD2000x1000

 

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Amino-Ficoll

As a T-cell independent carrier (TI carrier), coupling Ficoll with haptens has been shown to produce robust antigen-specific primary immune memory. Our method for amino functionalization yields stable primary amine groups. We carefully control the amine:polymer ratio and process to remove excess amine reagent. Custom Ficoll preparations are available upon request. GE Healthcare’s trademark Ficoll is a highly branched, 400kDa, hydrophilic polysaccharide widely used for blood separation.

Code Name Size
AFic-400×100 100 Amines/400kDa Ficoll 100 mg, 500 mg
AFic-400×150 150 Amines/400kDa Ficoll 100 mg, 500 mg
AFic-400×300 300 Amines/400kDa Ficoll 100 mg, 500 mg

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Antibody-Dextran Conjugates

Crosslinking membrane IgD on B cells with unconjugated anti-IgD antibody induces B cell proliferation at µg/ml concentrations. By contrast, anti-IgD dextran induces vigorous B cell proliferation at ng/ml. Furthermore, in contrast to unconjugated anti-IgD, anti-IgD-dex-activated B cells undergo substantial Ig class switching and differentiation into Ig-secreting cells in the presence of various second signals, including cytokines and Toll-like receptor ligands. Thus, Anti-IgD-dex is an efficient and potent polyclonal B cell activator for studying a wide range of B cell functions.

Fina Biosolutions provides anti-IgD and anti-IgM dextran conjugates, targeting mouse and human.

Streptavidin-dextran can be used to prepare multivalent polymer conjugates using a biotinylated species (i.e., antibody, TCR, antigen, etc.)

Product # Product Concentration size
FinaBio#0001  Anti-IgD dextran (mouse) 100 ug/ml 10 ug
FinaBio#0002 Anti-IgD dextran(human) 100 ug/ml 10 ug
FinaBio#0003  Anti-IgM dextran (mouse) 100 ug/ml 10 ug
FinaBio#0004 Anti-IgM dextran(human) 100 ug/ml 10 ug
FinaBio#0005 Human IgG dextran 100 ug/ml 10 ug
FinaBio#0006  Anti-IgD (Fab2) dextran (mouse) 100 ug/ml 10 ug
FinaBio#0007 Anti-IgD Dextram (Rat) 100 ug/ml 10 ug
FinaBio#0008 Anti-IgG Fc-gamma (human) 100 ug/ml 10 ug
FinaBio#0101 Streptavidin dextran 100 ug/ml 100 ug
FinaBio#1000A Custom Dextran Conjugates Inquire Inquire
FinaBio#1000B Bulk and quantiy orders Inquire Inquire

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Fluorescent Dextrans

Fluorescent dextrans have a wide variety of applications. The high water solubility, biocompatibility and range of functionalities make fluorescent dextrans ideal for many applications. They come in an array of molecular weights, fluorescent dyes, dye ratios and additional functional groups. Fluorescent dextrans from Fina Biosolutions use our well-characterized amino dextrans as their base. Our proprietary end-labeling chemistry allows us to synthesize unique multi-functionalized fluorescent dextrans.

“Fixable” fluorescent dextrans (fluorescent AmDex)

Product # Product Form Size
Flu-Amdex3.5K Fluorescein-Amdex 3.5K Lyophilized 25 mg
Flu-Amdex6K Fluorescein-Amdex 6K Lyophilized 25 mg
Flu-Amdex10K Fluorescein-Amdex 10K Lyophilized 25 mg
Flu-Amdex20K Fluorescein-Amdex 20K Lyophilized 25 mg
Flu-Amdex40K Fluorescein-Amdex 40K Lyophilized 25 mg
Flu-Amdex70K Fluorescein-Amdex 70K Lyophilized 25 mg
TMR-Amdex3.5K TMR-Amdex 3.5K Lyophilized 25 mg
TMR-Amdex6K TMR-Amdex 6K Lyophilized 25 mg
TMR-Amdex10K TMR-Amdex 10K Lyophilized 25 mg
TMR-Amdex20K TMR-Amdex 20K Lyophilized 25 mg
TMR-Amdex40K TMR-Amdex 40K Lyophilized 25 mg
TMR-Amdex70K TMR-Amdex 70K Lyophilized 25 mg

Fluorescent dextrans

Product # Product Form Size
Flu-Dex3.5K Fluorescein-dextran 3.5K Lyophilized 100 mg
Flu-Dex6K Fluorescein-dextran 6K Lyophilized 100 mg
Flu-Dex10K Fluorescein-dextran 10K Lyophilized 100 mg
Flu-Dex20K Fluorescein-dextran 20K Lyophilized 100 mg
Flu-Dex40K Fluorescein-dextran 40K Lyophilized 100 mg
Flu-dex70K Fluorescein-dextran 70K Lyophilized 100 mg
Flu-Dex250K Fluorescein-dextran 250K Lyophilized 100 mg
Flu-dex500K Fluorescein-dextran 500K Lyophilized 100 mg
TMR-dex3.5K TMR-dextran 3.5K Lyophilized 100 mg
TMR-dex6K TMR-dextran 6K Lyophilized 100 mg
TMR-dex10K TMR-dextran 10K Lyophilized 100 mg
TMR-dex20K TMR-dextran 20K Lyophilized 100 mg
TMR-dex40K TMR-dextran 40K Lyophilized 100 mg
TMR-dex70K TMR-dextran 70K Lyophilized 100 mg
TMR-dex250K TMR-dextran 250K Lyophilized 100 mg
TMR-dex500K TMR-dextran 500K Lyophilized 100 mg

MonoFluorescent Dextrans

Product # Product Form Size
mFlu-dex1.5K mono fluorescein-dextran 1.5K Lyophylized 2 mg, 25 mg
mFlu-dex3.5K mono fluorescein-dextran 1.6K Lyophylized 2 mg, 25 mg
mFlu-dex6K mono fluorescein-dextran 1.7K Lyophylized 2 mg, 25 mg
mFlu-dex10K mono fluorescein-dextran 1.8K Lyophylized 2 mg, 25 mg
mFlu-dex40K mono fluorescein-dextran 1.9K Lyophylized 2 mg, 25 mg
mFlu-dex70K mono fluorescein-dextran 1.10K Lyophylized 2 mg, 25 mg
mFlu-Dex250K mono fluorescein-dextran 1.11K Lyophylized 2 mg, 25 mg
mFlu-Dex500K mono fluorescein-dextran 1.12K Lyophylized 2 mg, 25 mg

 

MonoTMR Dextrans

Product # Product Form Size
mTMR-dex1.5K mono TMR-dextran 1.5K Lyophylized 2 mg, 25 mg
mTMR-dex3.5K mono TMR-dextran 3.5K Lyophylized 2 mg, 25 mg
mTMR-dex6K mono TMR-dextran 6K Lyophylized 2 mg, 25 mg
mTMR-dex10K mono TMR-dextran 10K Lyophylized 2 mg, 25 mg
mTMR-dex40K mono TMR-dextran 40K Lyophylized 2 mg, 25 mg
mTMR-dex70K mono TMR-dextran 70K Lyophylized 2 mg, 25 mg
mTMR-Dex250K mono TMR-dextran 250K Lyophylized 2 mg, 25 mg
mTMR-Dex500K mono TMR-dextran 500K Lyophylized 2 mg, 25 mg

 

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Mono-functionalized Dextrans

Monoamino dextrans and monocarboxyl dextrans are prepared by proprietary methods yielding a stable, monofunctionalized dextran, with a single functional group on the end of the polymer. The molecular weight profile is essentially unchanged, as determined by analytical size exclusion chromatography. Extensive processing and testing is performed to ensure that nonpolymer reagent is removed. This approach results in a consistent product with minimal lot-to-lot variation.

Monothiol dextran is synthesized with a single protected thiol (Dithiolpyridyl group).

These monofunctionalized dextrans can be used much like PEG—it can be used to increase protein half-life, reduce immunogenicity, and passivate surfaces. They can be labeled with a single fluorescent group to make a well-defined tracer.

 

Monoamino dextran

Product # Product Form Size
mAD1.5 Monoamino dextran 1.5K lyophylized 100 mg, 500 mg
mAD3.5 Monoamino dextran 3.5K lyophylized 100 mg, 500 mg
mAD6 Monoamino dextran 6K lyophylized 100 mg, 500 mg
mA10 Monoamino dextran 10K lyophylized 100 mg, 500 mg
mAD40 Monoamino dextran 40K lyophylized 100 mg, 500 mg
mAD70 Monoamino dextran 70K lyophylized 100 mg, 500 mg
mAD110 Monoamino dextran 110K lyophylized 100 mg, 500 mg
mAD250 Monoamino dextran 250K lyophylized 100 mg, 500 mg
mAD500 Monoamino dextran 500K lyophylized 100 mg, 500 mg
mAD2000 Monoamino dextran 2000K lyophylized 100 mg, 500 mg

 

Monocarboxyl dextran

Product # Product Form Size
mCD1.5 Monocarboxyl dextran 1.5K lyophylized 100 mg, 500 mg
mCD3.5 Monocarboxyl dextran 3.5K lyophylized 100 mg, 500 mg
mCD6 Monocarboxyl dextran 6K lyophylized 100 mg, 500 mg
mCD10 Monocarboxyl dextran 10K lyophylized 100 mg, 500 mg
mCD40 Monocarboxyl dextran 40K lyophylized 100 mg, 500 mg
mCD70 Monocarboxyl dextran 70K lyophylized 100 mg, 500 mg
mCD100 Monocarboxyl dextran 100K lyophylized 100 mg, 500 mg
mCD250 Monocarboxyl dextran 250K lyophylized 100 mg, 500 mg
mCD500 Monocarboxyl dextran 500K lyophylized 100 mg, 500 mg
mCD2000 Monocarboxyl dextran 2000K lyophylized 100 mg, 500 mg

Monobiotin dextran

Product # Product Form Size
mBiotDex1.5 Monobiotin dextran 1.5K Lyophylized 2 mg, 25 mg
mBiotDex3.5 Monobiotin dextran 3.5K Lyophylized 2 mg, 25 mg
mBiotDex6 Monobiotin dextran 6K Lyophylized 2 mg, 25 mg
mBiotDex10 Monobiotin dextran 10K Lyophylized 2 mg, 25 mg
mBiotDex40 Monobiotin dextran 40K Lyophylized 2 mg, 25 mg
mBiotDex70 Monobiotin dextran 70K Lyophylized 2 mg, 25 mg
mBiotDex250 Monobiotin dextran 250K Lyophylized 2 mg, 25 mg
mBiotDex500 Monobiotin dextran 500K Lyophylized 2 mg, 25 mg

Monothiol dextran

Product # Product Form Size
Monothiol dextran Monothiol dextran Custom lyophylized 100 mg

 

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Multi-functionalized Dextrans

We can provide multi-functionalized dextrans as custom products. These products are based on our proprietary chemistry for end-labeling dextrans.

The diagram below shows some of the types of multi-functionalized dextrans that we can synthesize.

As there are many combinations, most of our multi-functionalized dextrans are offered as custom products. Please inquire. We appreciate your suggestions for useful new products.

Mono functionalized- amino dextran
Examples: amino dextran containing a single carboxyl, thiol, biotin, squamate ester, click reagent, etc.

Mono functionalized carboxy dextran
Examples: carboxy dextran containing a single amino, thiol, biotin, squamate ester, click reagent, etc.

Di-functionalized dextrans containing exactly 1 functional groups on each end. The functional groups can be the same or different
Examples: Dextrans containing exactly 2 amines, carboxyls, etc.

 

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Specialty immunoreagents

Fina Biosolutions’ Specialty Immunoreagents are a collection of reagents mainly of interest to immunologists. These reagents arose out of both our own research and requests from other scientists. Please let us know if there is a reagent you would like us to make.

TNP-Dextran and TNP-Ficoll are classic T-cell independent model antigens. Due to the wide available of TNP immunoreagents, they serve as useful models of natural T-cell independent antigens like polysaccharides. TNP-OVA and TNP-BSA serve as T-cell dependent model antigens with the trinitrophenyl epitope. The set of TNP reagents also allows for the selection of a suitable ELISA antigen.

Our phosphorylcholine (PC) conjugates are based on the method of Chesboro and Metzger utilizing p-diazonium phenylphosphorylcholine.

Biotin labeled heparin is used in the identification of heparin binding sites. Our heparin conjugation utilizes the terminal end of heparin polymer resulting in minimum modification of the heparin.

Trinitrophenol

Product # Product Form Size
TNP-BSA-01 TNP-Bovine Serum Albumin lyophylized 10 mg, 50 mg, 100 mg
TNP-OVA-01 TNP-Ovalbumin lyophylized 10 mg, 50 mg, 100 mg
TNP-Ficoll-01 TNP-Ficoll(400) lyophylized 10 mg, 50 mg, 100 mg
TNP-Dextran-01 TNP-Dextran(T2000) lyophylized 10 mg, 50 mg, 100 mg

 

Phosphorylcholine

Product # Product Form Size
PC-BSA-01 PC-Bovine Serum Albumin lyophylized 10 mg, 50 mg
PC-OVA-01 PC-Ovalbumin lyophylized 10 mg, 50 mg

Phenol-arsonate

Product # Product Form Size
pARS-BSA-01 pARS-Bovine Serum Albumin lyophylized 10 mg, 50 mg
pARS-OVA-01 pARS-Ovalbumin lyophylized 10 mg, 50 mg

 

Heparin

Product # Product Form Size
mBiotin-Hep-01 MonoBiotin-Heparin lyophylized 1 mg, 10 mg
BSA-Hep-01 Heparin-BSA Lyophylized 1 mg, 10 mg
mA-Hep-01 Mono amino Heparin Lyophylized 1 mg, 10 mg

 

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Please contact us for your any inquiries, questions and information requests.

Tokyo Future Style, Inc.

mail

TEL : +81-29-851-9222

FAX : +81-29-851-9220

 

 

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For the development of human life science | Future-oriented Tradingcompany | Global marketing | Tokyo Future Style, Inc.