ACROBiosystems – Fc Receptor Proteins

INTRODUCTION

FcRn1

The efficacy of a therapeutic antibody not only depends on the Fab fragment and its binding activity to the target antigen, but also depends on the Fc fragment and its interaction with key Fc receptors. The binding affinity of the Fc fragment towards FcRn (FCGRT&B2M) would predict the antibody’s half-life, while that between the Fc fragment and FcγRIIIa (CD16a) would influence the antibody’s ability to elicit ADCC (antibody dependent cell mediated cytotoxicity). Therefore, candidates must be tested against a panel of receptors during antibody engineering.

ACROBiosystems offers a comprehensive collection of recombinant Fc receptor proteins, including their common variants, to help expedite your mAb development.

Product List (Please refer to the Complete Product List (EXCEL)) for more information)

FcRn / FCGRT & B2M
FCM-H5286 FcRn / FCGRT & B2M Heterodimer Protein
FCM-H8286 Biotinylated FcRn / FCGRT & B2M, ultra sensitivity (primary amine labeling)
FCM-H82W4 Biotinylated FcRn / FCGRT & B2M, Avi Tag (Avitag™)
FCM-M52W2 FcRn / FCGRT & B2M Heterodimer Protein
FCM-M82W6 Biotinylated FcRn / FCGRT & B2M, Avi Tag (Avitag™)
FCM-R5287 FcRn / FCGRT & B2M Heterodimer Protein
FCM-R82W7 Biotinylated FcRn / FCGRT & B2M, Avi Tag (Avitag™)
FCM-C5284 FcRn / FCGRT & B2M Heterodimer Protein
FCM-C82W5 Biotinylated FcRn / FCGRT & B2M, Avi Tag (Avitag™)
FCM-P5280 FcRn / FCGRT & B2M Heterodimer Protein
Fc gamma RIIIA / CD16a
CD8-H52H4 FCGR3A / CD16a (V158) Protein
CDA-H5220 FCGR3A / CD16a (F158) Protein
CDA-H82E8 Biotinylated Fc gamma RIIIA / CD16a (F158), Avi Tag (Avitag™)
CDA-H82E9 Biotinylated Fc gamma RIIIA / CD16a (V158), Avi Tag (Avitag™)
CDA-M52H8 FCGR3A / CD16a Protein
Fc gamma RIIIB / CD16b
CDB-H5222 Fc gamma RIIIB / CD16b Protein
CDB-H82E1 Biotinylated Fc gamma RIIIB / CD16b, Avi Tag (Avitag™)
Fc gamma RIIA / CD32a
CD1-H5223 FcGR2A / CD32a (H131) Protein
CDA-H5221 Fc gamma RIIA / CD32a (R131) Protein
CDA-H82E6 Biotinylated Fc gamma RIIA / CD32a (H131), Avi Tag (Avitag™)
CDA-H82E7 Biotinylated Fc gamma RIIA / CD32a (R131), Avi Tag (Avitag™)
Fc gamma RIIB / CD32b
CDB-H5228 Fc gamma RIIB / CD32b Protein
CDB-H82E0 Biotinylated Fc gamma RIIB / CD32b, Avi Tag (Avitag™)
CDB-M52H7 Fc gamma RIIB / CD32b Protein
CDB-M82E8 Biotinylated Fc gamma RIIB / CD32b, Avi Tag (Avitag™)
Fc gamma RI / CD64
FCA-H52H2 Fc gamma RI / CD64 Protein
FCA-H82E2 Biotinylated Fc gamma RI / CD64, Avi Tag (Avitag™)
CD4-M5227 Fc gamma RI / CD64 Protein
CD4-M52E8 Biotinylated Fc gamma RI / CD64, Avi Tag (Avitag™)
CD23  
CD3-H5249 CD23 / Fc epsilon RII Protein

 

 
Properties

 Authentic Human Cell-Expressed Proteins

 High Quality Materials

The consistency in purity and bioactivity is essential in the successful production of biotinylated proteins. All our proteins are evaluated by a variety of methods throughout the production process to ensure all relevant requirements are met.

  1. Purity greater than 95% by SDS-PAGE and SEC-HPLC
  2. Bioactivity validated in functional ELISA
  3. Endotoxin less than 1EU/µg by the LAL method

 
FCM-H5286-HPLC
The purity of human FcRn (FCM-H5286) was greater than 95% as determined in a HPLC analysis

 Biotinylated Proteins Available

The use of biotin labeling can make your assay development much easier. We offer a variety of ready-to-use biotinylated Fc receptors. These proteins are produced using our in-house developed labeling techniques, which confers high bioactivity and minimal batch-to-batch variation.

 
binffin
Biotinylated Human FcRn / FCGRT & B2M, Avi Tag (Avitag™) (Cat.# FCM-H82W4) coupled to SA coated sensor chip can bind Herceptin with an affinity constant of 2.24 μM as determined in a SPR assay (Biacore 2000). The data is generally provided by Biaffin GmbH &Co KG, Germany

 Binding Activity Validated

The binding activity of our Fc receptors have been thoroughly evaluated by both SPR and ELISA analyses prior to launch.

 
FcRn-FCM
Immobilized human FcRn (FCM-H5286) can bind human IgG1 with an affinity constant of 1 μM as determined in a SPR assay (Biacore 2000). The data is generally provided by Biaffin GmbH &Co KG, Germany.

Please contact us for your any inquiries, questions and information requests.
Tokyo Future Style, Inc.
info@tokyofuturestyle.com
TEL:+81-29-851-9222 FAX:+81-29-851-9220

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